Western blot was performed using Anti-STAT4 Monoclonal Antibody (H.56.9) (Product # MA5-14976) and a 86kDa band corresponding to Signal transducer and activator of transcription 4 was observed across cell lines and tissues tested . Whole cell extracts (50 µg lysate) of Jurkat (Lane 1), HEK-293 (Lane 2), A549 (Lane 3), HeLa (Lane 4), Daudi (Lane 5), HL-60 (Lane 6), Raji (Lane 7), MCF7 (Lane 8), Mouse Testis (Lane 9), Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Relative expression was observed in cell lines as well as in tissues.